Homeopathic Drug improve Metabolic Abnormalities Induced by HAART in Mice | Chapter 12 | Modern Advances in Pharmaceutical Research Vol. 1

Aims: Highly Active Antiretroviral Therapy (HAART) has changed the natural history of HIV infection, several adverse events may limit its efficacy. Antiretroviral drugs are associated with increased risk of severe hepatotoxicity. Homeopathy is a popular form of complementary and alternative medicine and is used to treat certain liver ailments. Chelidonium majus has a long history in the treatment of several diseases exhibit apoptotic activity, antioxidant and hepatic-protective effects. Current study evaluated the effect of homeopathic formulations of C. majus on metabolic alterations induced in mice subjected to HAART.

Methodology: Four-week old male Swiss Webster mice, weighing approximately 28-30 g, provided by the Central Animal Laboratory of the State University of Maringá, were used in the experiments. Five experimental groups with 10 animals each were distributed as follows: (I) animals treated with HAART diluted in 1.2 mL water gavage/day, (II) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus 6CH diluted  in water 1.0 mL once a day, added to the drinking water (1:10 mL) available ad libitum, (III) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus 12CH diluted in water 1.0 mL once a day, added to  drinking water(1:10 mL) available ad libitum, (IV) animals treated with HAART diluted in 1.2 mL water gavage/day + C. majus 30CH diluted in water 1.0 mL once a day, added to  drinking water (1:10 mL) available ad libitum, (V) non-treated animals (control group) received 1.2 mL water by gavage/day. The experimental groups were treated for 15 days. The drug in the form of mother tincture, prepared with the presses juice of the root of C. majus was mixed in equal parts of grain alcohol (PA) obtained from the  laboratory HNCRISTIANO, São Paulo, Brazil (lot 5387). The mother tincture was then diluted in 1×1012 water to obtain the homeopathic preparation 6CH, diluted in 1×1024 to obtain the homeopathic preparation 12CH and diluted in 1×1060 to obtain the homeopathic preparation 30CH. The method for drug preparation followed  the  Brazilian  Homeopathic  Pharmacopoeia.  The  dilution  was  considered  free  from  any toxicity.  Overall  clinical  evaluation  was  performed  and  serum  cholesterol,  triglycerides,  hepatic enzymes (AST and ALT) were assessed by specific methods. Results were analyzed with GraphPad Prism by Student ́s t test.

Results: Showed that the HAART group presented a weight gain lower (50%) than the control group.  Small little weight gain of animals using HAART may be related to the already known adverse effects of the antiretroviral. On the other hand, animals treated with C. majus regardless of concentration used  (6CH,  12CH  or  30CH)  presented  similar  weight  gain  when  compared  to  control.  Clinical parameters such as, body weight gain, postural pattern, piloerection and stress manipulation, results of treated animals showed that clinical C. majus had similar aspects to the control group not subjected to HAART. Results may indicate that C. majus induces a general clinical improvement in animals treated  with  HAART.  C.  majus  protects  the  liver  of  mice  from  possible  damage  caused  by antiretroviral therapy. ALT parameter showed levels which were 37.4% lower in mices treated with C. majus 6CH and 41% lower in mices treated with C. majus 30CH when compared to the group treated only with HAART. AST decreased in the group treated with C. majus 6Ch and 30CH demonstrate same levels of control.

Conclusion: Homeopathic preparations of Chelidonium majus, reduced the toxic effects of HAART in mice. Decrease in cholesterol  and triglyceride levels, higher weight gain and better AST and ALT levels  were  reported.  Evaluated  parameters  indicate  that  C.  majus  may  be  decreasing  HAART-induced hepatotoxicity.

Author(s) Details

N. A. Steiner
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.

A. L. P. P. Soares
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.

R. P. Regla
Department of Basic Health Sciences, StateUniversity of Maringá, Maringá, PR, Brazil.

M. Spack Jr
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.

A. R. T. Pupulin
Department of Basic Health Sciences, State University of Maringá, Maringá, PR, Brazil.

Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/47/240/405-1

View Volume: https://doi.org/10.9734/bpi/mapr/v1

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