Enzymatic Responses of Vigna radiata Seedlings under Biotic and Abiotic Stress | Chapter 13 | Theory and Applications of Microbiology and Biotechnology Vol. 2

Soil ecology is very fascinating due to its composition in which microbial strains sometime play a marvelous role in presence of several metals. The micronutrient metals are essential part of soil provides help in germination while toxic when exceed to a certain limits. This article aims to evaluate the cumulative effect of isolated bacterial strain of Pseudomonas stutzeri with copper (Cu) as a bioactive element in seed germination of four days old Vigna radiata. The germination of seeds also monitored at varying concentrations of Cu as a micronutrient. An important function of the enzyme lactate dehydrogenase (LDH) was observed in the shoots of four days old seedlings of V. radiata, cultivated in a series of experiments in the presence and absence of biotic and abiotic stresses simultaneously and separately. It was observed that germination of the seedlings were inhibited in presence of P. stutzeri separately may be due to the marked decrease in lipid contents. Results showed high LDH activity and high glucose contents as compared to the control ones indicated that high LDH activity was linked with elevated energy demand to overcome the stress for germination of seeds. Unexpectedly, germination of seeds was favored with increasing concentrations of Cu (100 ppm) with P. stutzeri simultaneously, although both biotic and abiotic seemed to be toxic separately. No microbial life at the highest concentration of Cu showed that it was toxic to the bacterial strain, but normal growth of seedlings suggested that dead mass of P. stutzeri was effective for the adsorption of the Cu on their surface due to which Cu mobility was checked recommencing the normal activity of LDH and glucose contents that believed to be at the cost of lipid contents. A suitable mechanism consistent with the finding has been proposed.

Author(s) Details

Prof. Dr. Rafia Azmat
Department of Chemistry, University of Karachi, 75270, Karachi, Pakistan.

Aliya Hayat
Department of Microbiology, Jinnah University for Women, 5C Nazimabad, Karachi, 74600, Pakistan.

Prof. Dr. Farha Aziz
Department of Biochemistry, Jinnah University for Women, 5C Nazimabad Karachi, 74600, Pakistan.

Prof. Dr. Masooda Qadri
Department of Chemistry, University of Karachi, 75270, Karachi, Pakistan.

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