Rapid and Mass Propagation of Hybanthus enneaspermus (L.) F. Muell. from Shoot Tip and Nodal Explants | Chapter 11 | Modern Research in Botany Vol. 1

A rapid and efficient protocol for in vitro propagation of Hybanthus enneaspermus (L.) F. Muell. (Violaceae) has been developed from the shoot tip and nodal explants. The explants were cultured on MS basal medium supplemented with different concentrations of cytokinins, viz., BAP and Kin, ranging from 5 µM to 25 µM, either individually or in combinations of both these cytokinins for shoot induction. Shoot buds of both the explants proliferated on MS medium supplemented with both cytokinins. The best response was observed on MS medium containing 15 µM BAP. Subsequently the optimum concentration of BAP (15 µM) was combined with different concentrations of Kin ranging from 2 µM to 10 µM. Maximum number of 28.6 ± 0.90  and 36.8 ± 1.54 shoots were produced on MS medium containing 15 µM BAP + 6 µM Kin from the shoot tip and nodal explants respectively. The regenerated shoots were transferred to rooting medium containing auxins at different concentrations ranging from 2 µM to 10 µM of IAA, IBA or NAA. The highest number of roots were observed on half strength MS medium fortified with 4 µM IBA. The plantlets were then hardened and acclimatized in soil. About 80% of plantlets were survived in the field condition. A completely randomized design was used in all experiments and analysis of variance and mean separations were carried out using Duncan’s Multiple Range Test.  Each treatment factor consists of 10 replicates repeated for 5 times. This protocol would help ex situ conservation of this medicinal plant.

Author(s) Details

Dr. P. Velayutham
Krishna College of Arts and Science, Kolluthannipatti, Karur Dt. Tamil Nadu, India.

C. Karthi
ICAR – National Research Centre for Banana, Tiruchirappali, Tamil Nadu, India.

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Use of Palm Male Inflorescence and River-Sand as Acclimatization Substrate for Plantain (Musa sp.) Cultivars | Chapter 07 | Advances and Trends in Biotechnology and Genetics Vol. 1

The objective of this work was to investigate the use of Palm Male Inflorescence (PMI) and river-sand as substrate for the acclimatization of plantain. Plantlets from three plantain cultivars (Batard, Ebanga and French Clair) were obtained after 16 weeks of tissue cultures and the plantlets were subjected to routine acclimatization under screen house conditions using two different substrates mixed in different ratios (100% Sand, 100% PMI, 75% PMI, 60% PMI and 50% PMI). The experiment was arranged in a completely randomized design with ten (10) replications; each replicate consisting of one micro-pot. The different substrates used significantly influenced the performance of the cultivars. The best medium for acclimatization for French Clair was 60% PMI in terms of percentage survival of plantlets (96.88%), plantlet height (6.03 cm), diameter (0.60 cm), number of leaves (4.42 leaves), leaf area (20.23 cm2), leaf emergence rate (1.64), number of roots (7.70 roots), and root length (18.86 cm). Ebanga plantlets had the  best results with 75% PMI in terms of percentage survival of plantlets (96.88%), plantlet height (6.18 cm), diameter (0.62 cm), number of leaves (4.39 leaves), leaf area (20.48 cm2), leaf emergence rate (1.76), and total fresh weight (10.05 g). Meanwhile with Batard cultivar, 50% PMI  was the best substrate in terms of percentage survival of plantlets (96.88%), plantlet height (4.41 cm), diameter (0.55 cm), number of leaves (4.55 leaves), leaf area (12.96 cm2), leaf emergence rate (1.55), and number of roots (5.73 roots). The relationship between the different variables assessed shows that plant height have a very strong positive correlation with pseudostem diameter (0.94), number of leaves (0.80), leaf area (0.98), and leaf emergence rate (0.77). This study clearly show that PMI can be a viable substrate to use with sand in plantlet acclimatization; however, the different plant cultivars had optimal result at different proportions of PMI. 

Author(s) Details

Ekwa Yawa Monono
Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, PMB 25 Buea, South West Region, Cameroon.

Jemimah Evenye Ngale
Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, PMB 25 Buea, South West Region, Cameroon.

Levai Lewis Dopgima
Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, PMB 25 Buea, South West Region, Cameroon.

Akongte Peter Njukang
Biotechnology Laboratory, Institute of Agricultural Research for Development (IRAD), Ekona, PMB 25 Buea, South West Region, Cameroon.

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View Volume: https://doi.org/10.9734/bpi/atbg/v1