Evaluation of Anti-diabetic Potentials of Methanol Extract of Ficus hispida Linn. Leaves against Alloxan Induced Diabetic Rats | Chapter 04 | Modern Advances in Pharmaceutical Research Vol. 3

Objective: The present study was designed to evaluate the acute and chronic effects of the methanol extract of Ficus hispida against alloxan (150 mg/kg) induced diabetic rats.

Methods: In acute study, hypoglycemic potency of methanol  extract of Ficus hispida was assessed by oral glucose tolerance test (OGTT) and in chronic study of 21 days, extract at different doses (ie 100, 200 and 400 mg/kg) was screened  for its anti-diabetic activity. Blood glucose level had been estimated at 0, 7th, 14th and 21st and addition to this serum concentrations of insulin, triglycerides, cholesterol, serum glutamate oxaloacetae transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and urea determined at 21st day of the study.

Results: In OGTT, standard glibenclamide and extract (200 and 400 mg/kg) have shown significant reduction in blood glucose level compared to control group. In chronic model, the methanol extract was effective in reducing the blood glucose levels (P <0.001) at higher dose (200 mg/kg and 400 mg/kg) and effect was comparable to that of standard. The extract could also significantly (P<0.001) reduced level of SGOT, triglycerides and cholesterol in serum and significantly (P<0.001) increased the insulin level in blood which proves beneficial antidiabetic potentials of the extract in diabetic model. The change in concentrations of SGPT and urea were not significant (P>0.05).

Conclusion: The methanol extract of Ficus hispida posses significant anti-diabetic activity in alloxan induced diabetis in rats model.

Author(s) Details

Dr. V. D. Ravichandra
Department of Pharmacognosy, East West College of Pharmacy, Bangalore-91, India.

Dr. Padmaa M. Paarakh
Department of Pharmacognosy, The Oxford College of Pharmacy, Bangalore-68, India.

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Combined Anti-diabetic Effects of Extracts of Artemisia annua var. Chiknensis (CBGE/CHNA/09/LTNGS/G) and Each of Three Other Plants (Momordica charantia Linn. Vernonia amygdalina Del. and Aegle marmelos Correa) Traditionally Used in Nigeria for the Treatment of Diabetes | Chapter 11 | Advances in Applied Science and Technology Vol. 6

The combined anti-diabetic effects of Extracts of Artemisia annua var. chiknensis with Laboratory code number (CBGE/CHINA/09/LTNGS/G), Momordica charantia Linn, Vernonia amygdalina Del. and Aegle marmelos Correa traditionally employed in Nigeria for the treatment of diabetes were studied. Fifty male albino rats which had been subjected to overnight fasting were rendered diabetic through single intraperitoneal alloxan injections (120 mg/kg body weight). They were then divided into 5 batches of ten rats each. The first batch was treated with A. annua leaf extract only. The second batch was treated with a combination of A. annua and M. charantia extracts. The third batch was treated with a combination of A. annua and V. amygdalina extracts while the fourth batch was treated with a combination of A. annua and Aegle marmelos extracts. The extracts were used in the ratio of 1:1 and treatment was done twice daily for a period of 30 days. The fifth batch of diabetic rats was not treated and thus served as control. The sixth batch of non-diabetic rats (10) was set up for comparison. Both the A. annua extract and its various combinations with the other experimental plant extracts resulted in insulin level enhancements and fasting blood glucose level reductions of the diabetic rats. There were significant differences (P≤0.05) in the insulin level of diabetic rats treated with A. annua leaf extract alone and those treated with different combinations of the plant extracts. The mean effects of the extracts on insulin and fasting blood glucose levels were most significant in A. annua + M. charantia (38.65 µlU/ml and 87.55 mg/dl) and A. annua + A. marmelos (38.55 µlU/ml and 87.92 mg/dl) treatments at P≤0.05. The non-treated diabetic rats had an average body weight of 94.90 g as compared to the original average body weight of 100 g at the commencement of the study. The treated diabetic rats had average body weight increments from 108.83g to 109.29 g. The non-diabetic rats had an average body weight of 114.10 g as compared to their initial average body weight of 100 g. The experimental plants were found to contain various biochemical constituents which were probably responsible for the blood serum insulin level enhancements and fasting blood glucose level reductions. The results obtained have shown that the A. annua leaf extract and its combinations with the other plant extracts could be employed in the management of hyperglycemia. The results have shown how effective A. annua var. chiknensis extract is in terms of lowering the blood glucose level or in the prevention of hyperglycemia. The results have also revealed that such blood glucose level reduction could be enhanced when the said extract is used in combination with other plant extracts that have similar effects in terms of management of hyperglycemia. It is hoped that new diabetes drugs that stem from the findings can be developed. However, in order to minimize the risk of diabetes cases in Nigeria, both conventional and traditional preventive measures should be considered.

Author(s) Details

Prof. Chike I. C. Ogbonna
Applied Microbiology and Biotechnology Research Unit, Department of Plant Science and Biotechnology, University of Jos, Nigeria.

Dr. (Mrs) Abigail. I. Ogbonna
Applied Microbiology and Biotechnology Research Unit, Department of Plant Science and Biotechnology, University of Jos, Nigeria.

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