Identification of the Essential Oils from Annona senégalensis Pers. Sous-espèce Oulotricha Le Thomas (Annonaceae) | Chapter 07 | Advances and Trends in Biotechnology and Genetics Vol. 3

Annona senegalensis Pers., Oulotricha Le Thomas subspecies (Annonaceae), is an evergreen shrub that is used for ethnomedicinal purposes and as a source of food. The chemical composition of the essential oils from leaves, stems bark, roots bark, epicarp and mesocarp of these plant, growing in Brazzaville (Congo), were analyzed by CG and CG-MS. These oils essentially contain sesquiterpenic compounds (58.3-97.7%), dominated by oxygenated sesquiterpenes (21.8-88.3%), with elemol (13.2-35.0%), β-and γ-eudesmols (3.7-58.3%) as characteristic components. The essential oils from roots and stems bark is distinguished by its high content in diterpenes (17.1 and 11.9% of the total), while the seeds presents a significant amounts of monoterpene hydrocarbons (25.8%) with α-pinene (6.2%) and β-phellandrene (11.5%) as major components accompanied by two oxygenated monoterpenes : bornyle acetate (4.5%) and smallest of 1,8-cineole. However, in the epicarp oil, the presence of about 5.8% of oxygenated monoterpenes as terpinen-4-ol and bornyle acetate in comparable rates (1.7%) was noted. The mesocarp oil is exclusively rich in aliphatic fatty acids (35.8%) which is absent in the other organs, but represented by lauric acid (18.0%), hexadecanoïc acid (8.6%), myristic acid (7.2%) and oleic acid (2.0%). Results were compared with same species collected in the democratic republic of Congo and in Cameroon essentially dominated by monoterpenes (84.2 and 87.6%).

Author(s) Details

Nkounkou-Loumpangou Célestine
Unit of Chemistry of Plant and of the Life, Faculty of Sciences and Technology, Marien Ngouabi University, B.P. 69, Brazzaville, Congo.

Gouollaly Tsiba1, Mahmout Yaya
Laboratory of Chemistry of Natural Substances, Faculty of Exact and Applied Sciences, University of N’Djamena, BP 1117, N’Djamena, Chad.

Elouma Ndinga Arnold Murphy
Unit of Chemistry of Plant and of the Life, Faculty of Sciences and Technology, Marien Ngouabi University, B.P. 69, Brazzaville, Congo.

Ouamba Jean-Maurille
Unit of Chemistry of Plant and of the Life, Faculty of Sciences and Technology, Marien Ngouabi University, B.P. 69, Brazzaville, Congo.

Chalchat Jean Claude
Heterocycle Chemistry Laboratory, Carbohydrate Chemistry, Essential Oil Chemistry, Blaise Pascal University. 4, avenue des Landais, Campus des Cézeaux, 63177, Aubière, France.

Figuérédo Gilles
Laboratory of Analysis of Plant Extracts and Flavors, Blaise Pascal University, 4, avenue des Landais, Campus des Cézeaux, 63177, Aubière, France.

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Phospholipase A2 Inhibition and Antiinflammatory Activity of F4 Fraction of Total Ethereal Leaf Extract of Annona senegalensis Pers. (Annonaceae) | Chapter 02 | New Insights on Chemical Research Vol. 1

Annona senegalensis Pers. (Annonaceae) is a plant which is used in african traditional medicine for the treatment of various diseases. This study aimed to investigate the analgesic and anti-inflammatory activity of total ethereal leaf extract fractions of A. senegalensis. Compounds of methanolic fractions of ethereal leaf extract of A. senegalensis were separated by gel sephadex chromatography, in five fractions (F1, F2, F3, F4, F5). Experiments were performed in acetic acid-induced contortions in mice, carrageenan rat paw edema and phospholipase A2 inhibitory test. The methanolic fraction of total ethereal leaf extract (10 mg/kg, per os) significantly prevented the carrageenan inflammatory edema. The variation of edema is 22.31±3.35%, 49.66±13.50%, 52.10±10.02% respectively at T1h, T3h and T5h. The increased edema after oral administration of F4 fraction administered at 300 µg/kg and 1 mg/kg per os is respectively 52.77±7.36% and 33.81±6.94%. The variation of edema in betamethasone group (1 mg/kg, per os) is 23.46±3.99%. F4 fraction at 300 µg/kg, significantly inhibited 16.39% of phospholipase A2 enzyme activity. F4 fraction (300 µg/kg, per os) also significantly prevented acetic acid-induced pain in mice. The number of abdominal contortions is 21 versus 72 in control group. F4 fraction compounds have a powerful analgesic and anti-inflammatory activity that involves phospholipase A2 inhibition, comparable to betamethasone profile on pain and inflammation.

Author(s) Details

M. Sene
Laboratoire de Pharmacologie et Pharmacodynamie, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

F. S. Barboza
Laboratoire de Pharmacologie et Pharmacodynamie, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

A. Ndong
Laboratoire de Pharmacologie et Pharmacodynamie, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

A. Sarr
Laboratoire de Pharmacognosie et Botanique, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

A. Wele3
Laboratoire de Chimie Organique et Thérapeutique, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

E. Bassene
Laboratoire de Pharmacognosie et Botanique, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

G. Y. Sy
Laboratoire de Pharmacologie et Pharmacodynamie, FMPO, UCAD, BP 5005 Dakar-Fann, Senegal.

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