Characterization and Antibacterial Activity of New -Aminoester Derivative, Synthesized via N-Alkyaltion of Methyl - Azido Glycinate| Chapter 9 | Theory and Applications of Chemistry Vol. 4

The amino ester derivative was synthesized via N-alkylation of methyl a-azido glycinate N-benzoylated 1 with methyl 2-amino-2-phenylacetate in methylene chloride and in presence of triethylamine as basic catalyst. The structure of the prepared compound was determined by spectroscopic methods: 1H-NMR, 13C-NMR, MS data, elemental analysis and confirmed by X-Ray diffraction. This compound was screened in vitro for its antibacterial activity against Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Salmonella enteric). The synthesized compound showed an interesting inhibitory effect against all the strains tested.

Author(s) Details

Hassane Faraj
Organic Chemistry Laboratory (LCO), Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.B. 2626, Fez 30000, Morocco.

Abdelilah El Hallaoui
Organic Chemistry Laboratory (LCO), Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.B. 2626, Fez 30000, Morocco

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Phytochemical Analysis and Antibacterial Efficacy of Mentha piperita (L) Ethanolic Leaf Extract against Clinical Isolates of Uropathogens | Chapter 10 | Theory and Applications of Microbiology and Biotechnology Vol. 1

Aim: The present study was designed to evaluate the antibacterial activity of Mentha piperita (L) leaf extract against clinical isolates of urinary tract infections.

Introduction: M. piperita L. (Peppermint) is a strongly scented herb belonging to family Lamiaceae. The plant is stimulant, aromatic and used for headache, vomiting and allaying nausea. In India the leaves are used to relieve sore throat. The most common form of bacterial infections is urinary tract infections (UTIs). They affect people of all age groups throughout their lifespan.

Methodology: The M. piperita ethanolic extract (MPEE) was prepared by cold maceration. The presence of phytoconstituents was determined using standard protocols. Clinical isolates of UTI pathogens such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were isolated from urine samples and identified by biochemical tests. The antibacterial property was determined by agar well diffusion method.

Results and Discussion: The preliminary phytochemical screening revealed the presence of amino acids, carbohydrates, alkaloids, proteins, glycosides, steroids, tannins and flavonoids. MPEE exhibited pronounced antibacterial activity against tested microorganisms. The extract exhibited antibacterial activity at 1000 µg concentration against S. aureus (21.50±1.22 mm), E. coli (19.33±0.81 mm), P. aeruginosa (15.33±1.69 mm) from high to low respectively. The minimum inhibitory concentration was ranged from 62.5 to 125 mg/ml.

Conclusion: The results of antibacterial studies confirm that MPEE was found to contain bioactive constituents that exhibited notable antibacterial activity. However, further isolation and characterization of phytoconstituents will be needed to evaluate the antimicrobial activities against a wider range of microbial pathogens.

Author(s) Details

Dr. Sowjanya Pulipati, B. Pharm, M. Tech (Bio-Tech), Ph.D
Vignan Pharmacy College, Vadlamudi- 522213, Guntur, Andhra Pradesh, India.

Sai Koushik Oruganti
Master of Medical Biotechnology, University of Windsor, Canada.

P. Srinivasa Babu, M. Parm, PhD
Vignan Pharmacy College, Vadlamudi- 522213, Guntur, Andhra Pradesh, India.

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