Biopreservation of a Cameroonian Indigenous Fermented Milk (Pendidam) Using Biosurfactants | Chapter 08 | Advances and Trends in Biotechnology and Genetics Vol. 3

Natural compounds with bio-preservative properties are gaining attention of researchers worldwide. It is the case of biosurfactants known as surface active compounds produced by microorganisms which deserved antimicrobial and antioxidant properties. The present study was aimed to improve the shelf life of a Cameroonian indigenous fermented milk “pendidam” using biosurfactants derived from that food matrix. Biosurfactants was extracted from pendidam, purified, characterized preliminarly and its in vitro antimicrobial activity against microorganisms isolated from pendidam was assessed. Then, the biosurfactants was applied in pendidam and the microbiological quality of pendidam was followed up during 4 days of preservation at room temperature. The results obtained show that the crude extract of biosurfactants exhibit emulsifying and surface activities which remained stable after heat treatment at 100°C for 15 min, NaCl concentration of 20% and pH values ranging from 2 to 12. Furthermore, the biosurfactants was microbicidal against Candida spp. and E. coli at a concentration of 100 mg/mL. Solution of Biosurfactants (50 mg/mL) introduced into pendidam at a ratio of 10% (v/v), reduced significantly (p<0.05) the proliferation of the total aerobic mesophilic flora, E. coli and Candida spp. during 4 days of preservation at room temperature while no adverse effects were noticed on the growth of lactic acid bacteria. The present study highlights the bio-preservative potential of biosurfactants and suggests its use in food industry as food preservatives.

Author(s) Details

Dr. Mbawala Augustin, Associate Professor
Department of Food Sciences and Nutrition, National School of Agro-Industrial Sciences, University of Ngaoundere, P.O.Box 455, Ngaoundere, Cameroon.

Dr. Pahane Mbiada Majesté, Ph.D
Department of Food Sciences and Nutrition, National School of Agro-Industrial Sciences, University of Ngaoundere, P.O.Box 455, Ngaoundere, Cameroon.

Dr. Mouafo Tene Hippolyte, Ph.D
Department of Food Sciences and Nutrition, National School of Agro-Industrial Sciences, University of Ngaoundere, P.O.Box 455, Ngaoundere, Cameroon.
Centre for Food and Nutrition Research, Institute of Medical Research and Medicinal Plants Studies, P.O.Box 6163, Yaounde, Cameroon.

Dr. Tatsadjieu Ngoune Léopold, Professor
Department of Food Engineering and Quality Control, University Institute of Technology, University of Ngaoundere, P.O.Box 455, Ngaoundere, Cameroon.

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Biological Studies of Newly Synthesized Benzo[d][1,2,3]triazole Derivatives | Chapter 06 | Theory and Applications of Chemistry Vol. 3

The study of heterocyclic systems provides most fascinating field for exploration in view of variety of interesting and intricate reactions, which lead to synthesis of unusual systems having diversities in their skeletal pattern. In this context, various new heterocycles and derivatives are synthesized with increased biological activities and less toxicity. Benzo-fused azoles containing three heteroatoms, specially, benzo[d][1,2,3]triazole, has received great attention, these days, for their chemical and biological properties and many of them are used as medicines for various diseases. Many researches have also shown that when heterocyclic and aliphatic secondary amines are introduced in a variety of heterocyclic compounds, the resulting compounds have been found to possess enhanced biological activities. Therefore aim of our research was to hook benzotriazole scaffold with these amines with a help of suitable linker in thirst of obtaining the resulting synthesized molecules possessing high and varied biological activities which can be useful to mankind in future. We have synthesized N-aminoalkylated derivatives of benzo[d][1,2,3]triazole. All the synthesized compounds were fully characterized on the basis of their detailed spectral studies and were evaluated for their antimicrobial activities in vitro.

Author(s) Details

Bharti Bhardwaj
Department of Chemistry, University of Delhi, Delhi-110 007, India.

Sunita Bhagat
Department of Chemistry, ARSD College, University of Delhi, India.

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Phytochemical and Antimicrobial Evaluation of the Essential Oil of Croatian Salvia brachyodon Vandas | Chapter 06 | Recent Advances in Biological Research Vol. 5

This study was designed to evaluate the phytochemical profile and the antimicrobial potential of the essential oil of Salvia brachyodon Vandas growing wild in Croatia. The emergence of multidrug resistant bacteria has become a serious threat to public health that has prompted research to the discovery and development of new active agents capable of partly or completely suppressing bacterial growth. Recently, a class of biological active compounds that has been attracting increased attention in drug discovery is the plant based derivatives. Many plants produce special biomolecules to protect themselves according to a stress or infection caused by microorganisms. Plants produce a broad assortment of secondary metabolites, including tannins, terpenoids, alkaloids, polyphenols and favonoids, which have been found in vitro to have antimicrobial properties against both Gram- positive and Gram- negative bacteria. Essential oils (EOs) are secondary metabolites, and are important for plant defense mechanism hence, they have various medicinal properties including antimicrobial activity. Natural products isolated from Salvia spp. have long been used in traditional medicine to treat several microbial afflictions, but considering the high number of species of this genus, the antimicrobial properties of all of them is not well known. Short tooth sage (S. brachyodon Vandas), an endemic species that grows in the south east areas of the Adriatic coast in Croatia, Bosnia and Herzegovina, and Montenegro. It is a perennial plant that grows up to 70-80 cm in height and flowers from July to September. The phytochemical components of the essential oil were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antimicrobial activity was assessed against a panel of representative Gram-positive and Gram-negative bacteria as well as fungi. The antimicrobial activities of the oil against pathogenic microorganisms were determined by using agar disc diffusion and broth microdilution methods. From the thirty-eight identified constituents representing 95.7% of the oil, 1,8-cineole (16.7%), β-pinene (19.7%) and α-pinene (7.6%), were the major components. The levels of oxygenated monoterpenes such as camphor (5.6%), borneol (4.2%), myrtenol (2.4 %) and terpinen-4-ol were significant. Other important compounds were sesquiterpenes hydrocarbons β-caryophyllene (6.6%), α-humulene (4.9%), viridiflorol (3.0%), spathulenol (2.9%) and aromadendrene. Preliminary antimicrobial screening revealed that the oil exhibited a very interesting antimicrobial profile. The oil exhibited moderate in vitro antibacterial activity after it was tested against twenty pathogenic bacteria and fungal strains, but high antimicrobial activity observed against medically important pathogens such as E. coli O157:H7, Listeria monocytogenes and Candida albicans. Results presented here may suggest that the essential oil of S. brachyodon possess antimicrobial properties, and is, therefore, a potential source of antimicrobial ingredient in food and pharmaceutical industry. The obtained results are preliminary and a further research is needed in order to obtain information regarding the practical effectiveness of essential oil to prevent the growth of foodborne and spoilage microbes under specific application conditions.

Author(s) Details

Dr. M. Skočibušić
Faculty of Science, University of Split, Croatia.

Mrs. S. Lacić
Faculty of Science, University of Split, Croatia.

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View Volume: https://doi.org/10.9734/bpi/rabr/v5