Medicinal Properties of Dacryodes edulis against Selected Clinical Bacterial Isolates | Chapter 04 | Current Research in Agriculture and Horticulture Vol. 1

The medicinal properties of Dacryodes edulis was investigated to determine the antimicrobial properties of the collected raw D. edulis pulp and seed extracts on some medically important human pathogens. The research was investigated against the selected human pathogens using standard microbiological and biochemical procedures. The D. edulis samples were harvested aseptically between the periods of April to June 2016 from its tree located at IBB way, Calabar Municipality, Nigeria. The aqueous and ethanolic extracts of the seed and pulp at varying concentrations of 1 g/10 ml, 2 g/10 ml, 4 g/10 ml, 5 g/10 ml was tested against some selected human pathogens such as Staphylococcus aureus, Klebsiella pneumonia, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris. The antimicrobial susceptibility results of the ethanol extracts of D. edulis seed showed marginally higher zones of inhibition to the clinical bacterial isolates tested than the ethanol extract of the pulp tested against the same clinical isolates. The organisms were resistant to the aqueous extracts of both the pulp and seed. The seed ethanol extracts showed a higher zone of inhibition of 21 mm against K. pneumoniae and 18 mm against P. vulgaris as compared to 18 mm as against K. pneumoniae and 17 mm against P. vulgaris showed by Gentamycin used as standard antibiotic control. The result of these has shown that D. edulis could be of immense importance in our nation’s young pharmaceutical industry for the development of new chemotherapeutic agent to address unmet therapeutic needs.

Author(s) Details

Prof. J. A. Lennox
Department of Microbiology, Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar, Nigeria.

Dr. B. E. Agbo
Department of Microbiology, Faculty of Biological Sciences, University of Calabar, P.M.B. 1115, Calabar, Nigeria.

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Thermophilic Bacteria: Novel Polymers for Biotechnological Applications | Chapter 01 | Advances and Trends in Biotechnology and Genetics Vol. 3

Aims: We analyzed thermotolerant bacterial isolates of thermophiles from various regions in South East coasts of India. They were analyzed for Fatty Acid Methyl Ester (FAME) for identification and classification.

Study Design: A total of forty three (9.6%) thermotolerant bacterial isolates of thermophiles were secluded out of 444 isolates obtained from water samples from the Adyar River, Marina coast, Muttu Kadu Backwaters, Kovalam coast (India) and a prawn hatchery. In addition, a novel thermotolerant Gram positive Bacillus type bacterium from the pelletized feed of fish was examined. A total of 18 thermotolerant bacteria were analyzed for Fatty Acid Methyl Ester (FAME).

Results: Amongst 134 isolates that grew on E. coli FAGI agar, 23 (17%) were thermotolerant when tested at 15 psi for 30 min. Of the 72 isolates that grew on Staphylococcus Baird Parker agar 10 (13%) were thermotolerant, whilst among the 128 isolates that grew on Salmonella agar ÖNÖZ 10 (8%) were thermotolerant. In contrast, none of the 110 isolates that grew on TCBS agar (Vibrio sp.) exhibited thermotolerance, although they were isolated from the same regions as the other bacteria. The optimal temperature for the growth of most thermophiles was 45°C, except for 9 isolates. Of the latter, 7 grew at 50°C, one at 52°C and one at 55ºC, respectively.There were some extremely thermotolerant bacterial isolates that exhibited consistency in profuse growth at 15 psi (121°C) for 30 min. Other isolates showed inconsistency in growth after autoclaving the cultures at 15 psi (121°C) for 30 min, and these bacteria showed development of endospores. Isolates of thermotolerant bacteria that grew on Staphylococcus Baird Parker agar and Salmonella agar ÖNÖZ were predominantly antibiotic resistant when compared with those bacterial isolates that grew on E. coli FAGI agar. A major 43 kDa toxin-protein as well as 15 other minor protein subunits was detected in the thermotolerant Bacillus sp. isolate. The proteins were toxic and caused mortality to goldfish Cyprin macrophthalmus. The thermotolerant bacteria were identified as B. cereus, Bacillus subtilis, B. pumilus, B. laevolacticus), B. alcalophilus, Kurthiasibirica, Geobacillus stearothermophilus, Staphylococcus schleiferi and S. gallinarum,

Conclusions: This study documents 43 thermotolerant bacterial isolates from water samples and a Bacillus-type bacterium from fish feed in Chennai region, India and these bacterial isolates offer the potential for exploitation in biotechnological applications.

Author(s) Details

Palaniappan Ramasamy
Research and Development Wing, Sree Balaji Medical College and Hospital, BIHER (Bharath University), Chromepet, Tamil Nadu, Chennai-600044, India.

Aruna Sharmili
Department of Biotechnology, Stella Mary’s College, Tamil Nadu, Chennai-600032, India.

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