Thermal Stabilization of HEWL by Adsorption on Biochar | Chapter 11 | New Advances in Materials Science and Engineering Vol. 2

The heat stress tolerance of hen egg white lysozyme (HEWL) was markedly enhanced by the adsorption of HEWL on bamboo charcoal powder (BCP), which is a kind of biochar. HEWL was firmly adsorbed on BCP even at high temperatures. The secondary structure of HEWL was altered to some extent by the adsorption of HEWL on BCP. The remaining activity of BCP-adsorbed HEWL exhibited more than 20% after the incubation for 30 min at 90°C although that of free one was hardly observed. Moreover, the half-life of BCP-adsorbed HEWL was 13 min at 90°C while that of free one was 4 min.

Author(s) Details

Hidetaka Noritomi
Department of Applied Chemistry for Environment, Tokyo Metropolitan University, Tokyo, 192-0397, Japan.

View Book: http://bp.bookpi.org/index.php/bpi/catalog/book/123

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Effect of Solvent on Catalysis of Protease Adsorbed on Biochar in Organic Media | Chapter 10 | New Advances in Materials Science and Engineering Vol. 2

The adsorption immobilization of the serine protease α-chymotrypsin (α-CT) onto bamboo charcoal powder (BCP), which is a kind of biochar, improved the transesterification rate of N-acetyl-L-tyrosine ethyl ester (N-Ac-Tyr-OEt) with n-butanol (BuOH) in 9 organic solvents. Organic solvents strongly affected the catalysis of BCP-adsorbed α-CT. The transesterification rate of BCP-adsorbed α-CT was much superior to that of free α-CT in every organic solvent. Especially, the transesterification rate of BCP-adsorbed α-CT was about 760 times higher than that of free α-CT in n-butyl acetate.

Author(s) Details

Hidetaka Noritomi
Department of Applied Chemistry for Environment, Tokyo Metropolitan University, Tokyo, 192-0397, Japan.

View Book: http://bp.bookpi.org/index.php/bpi/catalog/book/123