Endophytic Microbiome Population from Moringa oleifera Leaves Collected in Three Localities at Pernambuco State, Northeast Brazil | Chapter 07 | Theory and Applications of Microbiology and Biotechnology Vol. 1

Aims: This work investigated the population density of endophytic microorganisms from Moringa oleifera leaves collected in three localities at the State of Pernambuco (northeastern Brazil): Urban (campus from the Federal University of Pernambuco, UFPE) and forest (botanical garden) areas at Recife city and an urban area (industrial district) at Caruaru city.

Place and Duration of Study: Department of Antibiotics and Department of Biochemistry from Federal University of Pernambuco, during four years.

Methodology: Sodium hypochlorite was used to disinfect the leaves, which were macerated in PBS buffer and separately sowed on seven culture media supplemented with antibacterial or antifungal agents.

Results: The majority of endophytes isolated were bacteria and the highest density was found in leaves from the forest area. Regarding to fungi isolation, there was no statistical difference between the density in leaves from UFPE campus and the botanical garden while no fungal isolates was obtained from leaves collected in Caruaru. The highest diversity of endophytes was found in the leaves from the botanical garden, with 111 different isolates. A total of 71, 94 and 50 bacterial isolates were obtained from leaves of UFPE campus, botanical garden and Caruaru, respectively. The number of fungal isolates were 17 (UFPE campus) and 12 (botanical garden).

Conclusion: In conclusion, the methodology employed in this work was effective for the isolation of endophytes; it is still worth to mention that climatic and geographical conditions may interfere in density and diversity of endophytes from M. oleifera leaves.

Author(s) Details

Igor Felipe Andrade Costa de Souza
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

Thiago Henrique Napoleão
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

Thales Henrique Barbosa de Oliveira
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

Kêsia Xisto da Fonseca Ribeiro de Sena
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

Patrícia Maria Guedes Paiva
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

Janete Magali de Araújo
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

Luana Cassandra Breitenbach Barroso Coelho
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, 50670-420, Recife, Brazil.

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Prospection of Endophyte Microorganisms from Bauhinia monandra Leaves with Mainly Identification of Actinobacteria | Chapter 06 | Advances and Trends in Biotechnology and Genetics Vol. 1

The present work aimed the prospection of microorganisms from Bauhinia monandra leaves, with the purpose to identify endophytics to obtain strains with possible biotechnological applications. B. monandra leaves, disinfected with hypochlorite solution, were macerated in phosphate buffered saline and seeded in ten culture media containing antibacterial or antifungal agents. The endophytic filamentous fungus strains detected belonged to the genera Penicillium, Curvullaria and Aspergillus. Non-filamentous endophyte bacteria were grouped in the genera Bacillus, Burkholderia, Enterobacter and strains of endophytic Actinobacteria were classified as Streptomyces and Nocardiopsis. The isolation of endophytic microorganisms with nine culture media revealed better bacteria development with L-arginine agar; inorganic salt starch agar and potato dextrose agar were superior to Actinobacteria and fungus strains, respectively. The present study revealed the predominance of the genus Penicillium in leaves of B. monandra. This work introduces the first data of identification from endophyte Actinobacteria in the leaves of B. monandra.

Author(s) Details

Rosilma de O. Araujo-Melo
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.

Thales Henrique B. de Oliveira
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.
Departamento de Bioquímica, Centro de Biociências, UFPE, Avenida Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

Carlos Vinícius J. de Oliveira
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.

Janete M. de Araújo
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.

Kêsia X. F. R. de Sena
Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco (UFPE), Avenida dos Economistas, S/N, Cidade Universitária, Recife-PE, CEP 52171-011, Brazil.

Luana Cassandra Breitenbach Barroso Coelho
Departamento de Bioquímica, Centro de Biociências, UFPE, Avenida Moraes Rego, S/N, Cidade Universitária, Recife-PE, CEP 50670-420, Brazil.

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View Volume: https://doi.org/10.9734/bpi/atbg/v1