Morphological Character Variations in Lasiodiplodia Species: Pathogen of Inflorescence Dieback in Cashew Growing Ecologies of Nigeria | Chapter 8 | Current Research Trends in Biological Science Vol. 1

Nine isolates of Lasiodiplodia theobromae were collected from cashew inflorescences showing typical symptoms of dieback disease in nine different farms belonging to various cashew growing ecologies of Nigeria. The result revealed that most of the Lasiodiplodia species isolates exhibited significant differences in morphology, colour and spore dimensions. The colony growth rate of Lasiodiplodia species range from 11.95 mm to 14.17 mm, colony texture and colour of the isolates in the obverse were fluffy dark mouse grey, fluffy mouse grey, fluffy olivaceous grey or fluffy groh grey while the reverse colour of the isolates was either greyish blue or sky grey. Sporulation was observed at varied degrees in all the Lasiodiplodia species isolates except in isolates from Oro and Ejule and likewise is the numbers of pycnidia produced varied in all the isolates across growing ecologies. Significant variations were observed in the characters and morphology of the Lasiodiplodia species isolates causing inflorescence dieback of cashew in Nigeria. Conidia of all isolates are septated with single septa but the septa sizes varies and conidia sizes also differ. Eigenvalues and variance proportion consistently decreased among selected characters and the proportional contribution of each character to the total variance also varied in dimension and quantity. Four clusters significantly evolved in the dendogram with 2, 2, 4 and 1 isolates within each cluster.

Author(s) Details

Dele Omoyele Adeniyi
Department of Plant Biology, University of Ilorin, P.M.B. 1515 Ilorin, Nigeria and Plant Pathology Section, Cocoa Research Institute of Nigeria, P.M.B. 5244, Ibadan, Nigeria.

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Morphological Characterization and Tissue Culture of Medicinal Plant Aloe vera L. | Chapter 14 | Advances and Trends in Biotechnology and Genetics Vol. 3

The morphological characterization and micro propagation were carried out on Aloe vera (Aloe barbadensis Miller) accessions collected from different locations in India were discussed in this book chapter. The experiments were conducted on 12 Aloe accessions and the leaf length, width, thickness, stem length and thickness were measured using verniercalipers. The plantlets were chemically treated with 2.5% (w/v) of sodium hypochlorite which is an alternative safer surface sterilization method for replacement of mercury chloride by using shoot tip as explants. The sterilization results indicate that the highest number (93%) of viable explants (10) with the best regeneration potential was in Murashige and Skoog (MS) medium compared with other treatments. Multiple shoots/explants (35) were found in MS medium containing 6-benzylaminopurine (BAP, 1.5 mgL-1; kinetin (0.5 mgL-1), indole-3-butyric acid (IBA, 0.2 mg L-1), indole-3-acetic acid (IAA, 0.1 mg L-1), and naphthalene acetic acid (NAA, 0.2 mg L-1). Based on the shoot proliferation, a maximum number (87.89%) of shoots obtained from the micro-shoot culture method and a maximum number (91.12%) of roots were obtained on MS medium supplemented with NAA (0.2 mg L-1), IBA (0.2 mgL-1) and IAA (0.1 mgL-1) which was found to be the best. The healthy rooted plantlets were successfully transferred and obtained as the plants were of 100% survival. The regenerated plants were morphologically similar to the mother plant.

Author(s) Details

B. Chandra Sekhar Singh
Department of Plant Science, College of Agriculture and Veterinary Sciences, Ambo University, Ambo, Post Box: 19, Ethiopia.

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