Antibacterial Activity of Polyphenolic Extracts from Different Algerian Honeys against Methicillin Resistant Staphylococcus aureus | Chapter 11 | Innovations in Medicine and Medical Research Vol. 1

The prevalence of patients infected with Methicillin resistant Staphylococcus aureus present different problems of nosocomial infections diseases in addition to multidrug resistance. This work aim to evaluate the antibacterial effect of four polyphenolic extracts from honey against Methicillin resistant Staphylococcus aureus. An extraction of polyphenol was carried out from different honey samples collected from different regions of the Algerian territory. First, the physicochemical, microbiological quality and pollen analysis was studied. Then, the evaluation of the antibacterial effect was performed by the determination of inhibition diameter and the minimal inhibitory concentration. The results clearly show the sensitivity of Methicillin Resistant Staphylococcus aureus against the polyphenolic extracts. The four samples presented an inhibitory effect and the polyphenolic extract of Jijel has the highest inhibitory effect among all extracts. This activity depend not only with the type of polyphenolic compounds but also with the botanical origin and the type of honey as honeydew honeys which is more effective than nectar honey.

Author(s) Details

Belkhodja Hamza
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, Algeria.

Belmimoun Asmaa
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, Algeria.

Pr. Meddah Boumediene
Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, Algeria.

View Volume: http://bp.bookpi.org/index.php/bpi/catalog/book/139

Antibacterial Activity of Flavonoids Extracted from Seeds of Pongamia pinnata Linn against Methicillin Resistant Staphylococcus aureus | Chapter 08 | Recent Advances in Biological Research Vol. 3

Introduction: Methicillin resistant Staphylococcus aureus (MRSA) is defined as the resistant to penicillinase-stable penicillin’s, thus the acronym MRSA is still under use even though methicillin is no longer the agent of choice for treatment. The use of vancomycin for MRSA remains as the treatment of choice but concerns with rising resistance to glycol peptides call for the restrictive use of these drugs. The resistance mechanism and the genes that mediate resistance have presumably evolved in organism that produce antibiotics such that the antibiotic produced is not effective against the producing organism.

Aims: To assess the antibacterial property of seed crude extracts of Pongamia pinnata Linn and isolated flavonoids component from crude extract against Methicillin resistant Staphylococcus aureus obtained from clinical isolates.

Study Design: Observational study.

Place and Duration of the Study: Department of Allied health sciences, Department of Biochemistry and Department of Microbiology in Sri Devaraj Urs Academy Of Higher Education and  Research, Tamaka, Kolar, between February 2014 and march 2015.

Methodology: Confirmed clinical isolates for MRSA were collected from Microbiology department to test the efficacy of crude extracts of seeds from Pongamia pinnata L. Methanolic crude extract has been preferably used for isolation of flavonoid content using Dimethyl Sulfoxide [DMSO] and methanol as ideal solvents during extraction process by column chromatography technique. Agar well diffusion method was performed to determine the antibacterial activity of crude seed extracts of Pongamia pinnata and isolated flavonoids by using quercitin as positive control for flavonoids. Vancomycin a glycopeptide powder used as gold standard for comparing bactericidal activity of quercitin, flavonoids and crude extracts of P. pinnata on MRSA.

Results: The highest antibacterial activity (75-89%) was observed in crude extract of Pongamia pinnata in comparison to vancomycin considered as cent percent. Extracted flavonoids showed activity (66-92%) with respect to crude extract and (50-84%) with vancomycin and the activity (71-92%) with respect to quercitin when tested with concentration ranging from 25-400 µg/ml.

Conclusion: This study showed that seed extracts of Pongamia pinnata L and its phytochemical compound flavonoids showed potential antibacterial activity against MRSA using quercitin and vancomycin. Flavonoids occupy the first grade antimicrobials in combating methicillin resistant staphylococcus aureus infections. These infections which are prominent in ICU units and HICU units can be drastically controlled without any side effects.

Author  Details:

Mary Shobha Rani Inala

Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

C. D. Dayanand

Department of Biochemistry/Allied Health Sciences, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

P. M. Beena

Department of Microbiology, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

A. V. M. Kutty

Department of Biochemistry/Allied Health Sciences, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.

Read full article: http://bp.bookpi.org/index.php/bpi/catalog/view/50/400/430-1

View Volume: https://doi.org/10.9734/bpi/rabr/v3