Response of Pseudomonas Species from Contaminated Soils to Selected Organic (Synthetic) Pesticides | Chapter 15 | Theory and Applications of Microbiology and Biotechnology Vol. 1

Growth response profile of three species of Pseudomonas isolated from pesticide contaminated soils within Uyo, Nigeria was studied using standard microbiological and analytical techniques. The ability of the isolates to tolerate varying concentrations of commercially available pesticides namely; Decis, DD force and Cyperforce was assessed over a 96 hour period. Selective enrichment cultures with graded concentrations of the pesticides were used to assay their growth response profile and the absorbance determined using CO75 digital colorimeter. The results showed that the Pseudomonas species differed biochemically. Their growth response at pesticide concentrations 0.0001, 0.001, 0.01, 0.1, 1 and 10% v/v differed significantly (P<0.05) at 24 hours interval for four days. At 10% concentration of Decis pesticide over 96 hour, the absorbance were 0.20, 0.23 and 0.30 for Pseudomonas from Agriculture research farm, hospital dumpsite and municipal waste dump site respectively. This ability therefore offers a veritable tool for use in the bioremediation and ultimate restoration of pesticide contaminated soils but however requires further evaluation.

Author(s) Details

Uduak U. Ndubuisi-Nnaji
Department of Microbiology, University of Uyo, Uyo, Nigeria.

Utibe A. Ofon
Department of Microbiology, University of Uyo, Uyo, Nigeria.

Godwin E. Udofia
Department of Microbiology, University of Uyo, Uyo, Nigeria.

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Bio-pesticidal Properties of Neem (Azadirachta indica) | Chapter 03 | Advances and Trends in Agricultural Sciences Vol. 1

Consequence upon the geometrically rising world population and the increasing pressure on food items, it has become increasingly necessary to increase food production from the present level. The possibility of achieving this is not only to increase production but also to protect the crops cultivated. Crop protection can be achieved through several means. One of such is the use of pesticides. This paper therefore reviews the use of neem extracts as bio-pesticides among other plant species with inherent pesticidal activities. It is no doubt that the chemical pesticides or insecticides possess inherent toxic substances that endangers the ecological environment, operators of application equipment, soil microbiota and consumers of the agricultural products. It is therefore important that we encourage the use of biological pesticides as they affect only target pest, are easily biodegradable, increase farm land fertility, environmentally friendly, cost effective and ease of availability. It is also important that because of the low cost of production of bio-pesticides it should be encouraged as an option in African countries especially Nigeria in agricultural practices. The practice of farmers making their own neem-based products for pest control would reduce their dependence on external inputs for agriculture. It would also reduce their cost of pest control to almost zero, leaving only labour as a potential expenditure item. Pests can also be controlled without the use of toxic chemical pesticides, which will reduce the harm posed to humans and the environment alike. There is wide scope for innovation in developing neem as an efficient bio-pesticide. There is enough information to encourage the use of different neem extracts. With the increasing trend of using bio fertilizers, insecticides and pesticides, neem should be increasingly cultivated and grown all over the world to get active ingredient-azadirachtin, responsible for stopping the growth cycle of pests. Neem is also assuming a lot of importance in crop management. Considering the fact that neem is not only a cheaper, naturally occurring product and an effective method to control pests and insects, but also has no side effects on plants or other living beings especially soil micro biota.

Author(s) Details

Dr. Bassey Etta Agbo

Department of Microbiology, University of Calabar, P. M. B. 1115, Calabar, Nigeria.

Dr. (Mrs.) Abo Iso Nta

Department of Zoology and Environmental Biology, University of Calabar, P. M. B. 1115, Calabar, Nigeria.

Mr. Mathias Okang Ajaba

Department of Science Laboratory Technology, University of Calabar, P. M. B. 1115, Calabar, Nigeria.

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