Research on Evaluation of the Effect of Crude Extract of Datura innoxia on the Cardiovascular Action of Detomidine in Rabbits | Chapter 8 | Trends in Pharmaceutical Research and Development Vol. 1

Datura innoxia indigenous shrub in South Asia region belongs to the family Solanaceae which contains medicinal important alkaloids (hyoscine, atropine, hycosamine, etc). Thirty adult rabbits of mixed breed, male and female were pretreated with 0.1 mg/500 mg of crude extract (1% of dimethyl sulfoxide (DMSO) solution) and injected 5 min later with 0.01 mg/500 mg of detomidine (group A, n=15) or Saline (group B, n=15). Mean arterial pressure, measurements and electrocardiography were performed for 65 min. After injecting crude extract of D. innoxia, the heart rate was increased by 45 and 46.34% in groups A and B, respectively. Heart rate remained increased after the injection of detomidine returning to base line values after 15 min. No increase in the mean arterial  pressure (MAP) was noted in group B rabbits. Crude extract shortened PR and QT interval in both groups but after detomidine, PR and QT interval were enlarged significantly at the end of the experiment. The second degree atrioventricular was blocked in two rabbits after 40 min only in group B. It was concluded that alkaloid present in D. innoxia prevented detomidine induced Bradycardia  and might be useful during combination against the Bradycardia induced by alpha-2 agonist in the rabbits.

Author(s) Details Dr. S. U. Rehman
Faculty of Sciences, University of Central Punjab, Lahore, Pakistan.

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Influence of Dietary Supplementation of Coated Sodium Butyrate and/or Synbiotic on Growth Performances, Caecal Fermentation, Intestinal Morphometry and Metabolic Profile of Growing Rabbits | Chapter 09 | Research and Development in Agricultural Sciences Vol. 2

Aim: The aim of the present experiment was to study the synergistic effects of dietary supplementation with coated slow released sodium butyrate (CM3000®) and a commercial synbiotic (Poultry-Star®) on the productive performance and intestinal morphometry of the growing rabbits.

Study Design: Laboratory experimental design was used in this study.

Place and Duration of Study: The study was conducted in the experimental rabbitry of Physiology Department, Faculty of Veterinary Medicine Cairo University, Egypt. The s duration of the study persists for 70 days.

Methodology: Thirty- two apparently healthy male New Zealand rabbits with average body weight of 544 ± 9 g were divided randomly into four dietary treatments at weaning (28th day of age). The control group (C) was fed on standard basal diet with no supplementation. Rabbits in the second group (T1) received the same basal diet supplemented with CM3000® 500 g/ton feed. Animals in the third group (T2) consumed the basal diet containing Poultry-Star® 500 g/ton feed. Rabbits in the fourth group (T3) were fed on the basal diet enriched with mixture of CM3000® and Poultry-Star®, 250 g/ton feed for each. Feed and water were offered ad-libitum for 70 days experimental period. Body weight and feed consumption were recorded biweekly to calculate body weight gain and feed conversion. At the end of the experimental period blood and caecal content samples were collected from all animals. Ce rtain haematological metabolic parameters namely, glucose [1] triglycerides [2], total cholesterol [3] total protein [4], albumen and urea [5]. Caecal content samples were collected at the end of the experimental period post slaughtering for determination of caecal fermentation pattern namely, pH, total short chain fatty acids [6] individual volatile fatty acids [7]and ammonia concentration [8,9]. Duodenal tissue samples were collected for histomorphometry. The results revealed that additives used improved significantly live body weight compared to the control group. Rabbits in T3 group showed the highest body weight gain. In addition, supplementation of the basal diet with a mixture of additives revealed significant increase of feed intake. The blood urea level was reduced significantly in bucks of T1. The rabbits in T3 group recorded the highest level of blood glucose. Caecal pH revealed a significant decrease in T1 and T3. The mixture of additives has positive results on the intestinal morphometry.

Conclusion: Coated butyrate and are capable of improving performance, enhancing intestinal health.

Author(s) Details

Mr. Anthony Kodzo-Grey Venyo MB ChB FRCS(Ed) FRCSI FGCS Urol. LLM
Department of Urology, North Manchester General Hospital, Manchester, M8 5RB, United Kingdom.

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