Spilanthes paniculata Flower Extracts Attenuates Paracetamol Induced Liver Damage by Antioxidant Mechanism | Chapter 3 | Trends in Pharmaceutical Research and Development Vol. 1

The present study was undertaken to investigate the antioxidant and hepatoprotective effect of Spilanthes paniculata Wall. ex DC flower extracts against paracetamol-induced liver damage. The study was conducted in 36 male Wistar rats of either sex, and six groups were established. While the first group was maintained as normal control (NC, distilled water), Groups 2 to 6 were administered 3 g/kg Paracetamol (PAR) for 2 day, 100 mg/kg Silymarin (SMR), 500 mg/kg Methanolic extract (MESP), Petroleum ether extract (PEESP), Ethyl acetate extract of S. paniculata (EAESP) suspended in 0.5% tween 80 plus PAR, respectively. PAR was administered in the same schedule as in group 2, the treatment with silymarin and extracts was given for 10 days orally, respectively. It was observed that PAR significantly increased serum Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline phosphatase (ALP) activity liver MDA levels (P<0.01) and significantly decreased liver Glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) activity (P<0.01), when compared with the normal control group (NC). On the other hand, statistically significant (P<0.01) changes were observed in the biochemical parameters of the group which was administered SMR, PEESP and EAESP. Compared with the pathological changes observed in the liver in the form of congested sinusoids and centrilobular necrosis, in the group which was administered paracetamol alone (PAR), lesions were determined to be less severe particularly in the group (PEESP and EAESP). The               study shows that administration of PEESP and EAESP offered a therapeutic potential for the treatment of hepatotoxicity induced by paracetamol via regulation of endogenous antioxidant system in liver.  

Author(s) Details

Syed Ayaz Ali
Department of Pharmacology, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, Maharashtra, India.

Shukla Mahanand
Department of Pharmacology, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, Maharashtra, India.

Subur W. Khan
Department of Pharmacognosy, Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Rauza Bagh, Aurangabad, Maharashtra, India

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Effect of Cymbopogon citratus Stapf (DC) on Type 2 Diabetes Mellitus-induced Dyslipidemia: Current Knowledge | Chapter 11 | Current Trends in Medicine and Medical Research Vol. 3

Introduction: Diabetic dyslipidemia (DD)  is  a collection  of  quantitative,  qualitative and  kinetic  lipid abnormalities associated with diabetes mellitus that together caused the lipid profile to become more atherogenic.  It  consists  of  elevated  serum  concentration  of  triglyceride-cholesterol  (TG-C),  a  high serum level of small dense low density lipoprotein-cholesterol (sd LDL-C), low level of high-density lipoprotein-cholesterol (HDL-C) and normal to slightly elevated level of total low density lipoprotein-cholesterol (LDL-C).

Aims: Diabetic  dyslipidemia  is  a  recognized  risk  factor  for  coronary  heart  disease  (CHD). Plant medicinal  agents  such  as Cymbopogon  citratus (C.  citratus)  have  shown  potential  as alternative therapies for reducing cardiovascular risk factors. The aim of this study was to investigate the effect of C. citratus leaf extract on the atherogenic index of plasma (AIP) in diabetic dyslipidemic rats (n=35).

Materials and Methods: A C. citratus extract was prepared by ethanol extraction of leaf material. Rats were divided into seven groups (n=5) as follows: (a) Normal diet control, (b) Hyperlipidemic diet (HLD)  control,  (c)  HLD  +  65 mg/kg  streptozotocin  (STZ)  control  (d)  HLD  +  STZ  +  250mg/kg C. citratusextract (CCE), (e) HLD + STZ + 500mg/kg CCE, (f) HLD + STZ + 1000mg/kg CCE, and (g) HLD + STZ + 5mg/kg atorvastatin + 600μg/g glibenclamide. Animals were treated with HLD for 14 days and then injected intraperitoneally with 65mg/kg STZ. Confirmed diabetic dyslipidemic animals were  treated  intragastrically  with  CCE  at  doses  of  250,  500,  and  1000mg/kg,  with  5mg/kg atorvastatin, and with 600μg/g glibenclamide for 30 days.

Results: The extract, which tested positive for tannins, saponins, alkaloids, flavonoids, etc. lowered fasting  blood  glucose  and  glycosylated  hemoglobin  levels,  and  dose-dependently  decreased  the serum levels of T-chol, LDL, VLDL, and β-HMG-CoA reductase, while simultaneously increasing HDL levels. The AIP was lowered in a dose-dependent manner by 33, 43.7, and 52.4% in groups treated with 250, 500, and 1000 mg/kg of CCE respectively.

Conclusion: The  results  indicate  that  the C.  citrates extract  had  an  ameliorative  effect  on hyperglycemia, hyperlipidemia, obesity, and atherogenic index of plasma.

Author(s) Details

Christopher Edet Ekpenyong

Department of Human Physiology, Faculty of Basic Medical Sciences, University of Uyo, Nigeria.

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View Volume: https://doi.org/10.9734/bpi/ctmmr/v3