Harnessing of Proteomic and Molecular Analysis of Bali Cattle Saliva: Another Side to Study the Potential of Bali Cattle (Bos javanicus) as a Native Indonesian Cattle | Chapter 2 | Current Strategies in Biotechnology and Bioresource Technology Vol. 1

Bali cattle are native to Indonesia, with some superior properties such as high fertility, remarkable resistance to heat stress as well as to most diseases that lead Bali cattle are suitable for tropical regions such as Indonesia and may be for other tropical countries as well. Up to now studies have been focused on their main purpose as small beef cattle, in fact Bali cattle saliva has also shown potential as a source of biological material. This book chapter discusses the studies that have been carried out in the last five years regarding saliva of Bali cattle from proteomic and molecular aspects. From this discussion it is hoped that further thoughts will be born to explore the Bali cattle from other aspects. Hopefully the information is also useful to explore the benefits of saliva in other native animals besides Bali cattle. 

Author(s) Details

Sulaiman N. Depamede
Laboratory of Microbiology and Biotechnology, Faculty of Animal Sciences, University of Mataram, Jalan Majapahit No. 62, Mataram 83125, Indonesia.

Djoko Kisworo
Laboratory of Animal Product Processing Technology, Faculty of Animal Science, University of Mataram, Jalan Majapahit No. 62, Mataram 83125, Indonesia.

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Screening for Oral High-risk Human Papillomavirus (HPV) among a Pediatric Patient Population | Chapter 10 | Emerging Research in Medical Sciences Vol. 2

Introduction: Human papillomavirus (HPV) is a known cancer-causing virus that has been primarily linked to cervical cancers. Recent studies have shown that HPV may also induce cancer in other tissues, including oral epithelia and mucosa. To determine the presence of oral HPV infection among pediatric patients, salivary samples were assessed to determine oral prevalence of high-risk HPV.

Methods: Using existing pediatric saliva samples from pediatric clinic patients (aged 6 – 16 years), this retrospective study involved isolating DNA for PCR screening for both high-risk strains of HPV (HPV16, HPV18). A total of n=98 patient samples were available for analysis.

Results: Demographic analysis of these samples revealed the majority were derived from females (63.2%) and mostly from Hispanics (54.1%). DNA was successfully isolated from 95.9% of samples (n=94/98) with an average concentration of 142.5 ng/uL. PCR screening demonstrated only a subset of patient samples harbored high-risk strains, HPV16 (n=9) and HPV18 (n=6), which represents 15.9% of the total sample population – and were concentrated among the older patients (>13 years old).

Conclusions: Although this retrospective study does not have a sufficient sample size to provide more robust analysis of other risk factors (race, sex, sociodemographic), these results to demonstrate that oral HPV infection may be successfully detected among a pediatric population. As more studies demonstrate oral HPV infection in children, these data are of significant value to other dental, medical, oral and public health professionals who seek to further an understanding of oral health and disease risk in pediatric populations.

Author(s) Details

Douglas Bowen
Department of Advanced Education in Pediatric Dentistry, Las Vegas – School of Dental Medicine, University of Nevada, 1001 Shadow Lane, Las Vegas, Nevada, 89106, USA.

Dr. Karl Kingsley, (PhD, MPH)
Department of Biomedical Sciences, Las Vegas – School of Dental Medicine, University of Nevada, 1001 Shadow Lane, Las Vegas, Nevada, 89106, USA.

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