In vivo Antioxidant Activity of Sida cordifolia Linn. in K2Cr2o7 Induced Oxidative Stress by Measurement of Reactive Oxygen Species Levels in Rats | Chapter 09 | New Insights into Disease and Pathogen Research Vol. 3

Ayurvedic textbooks ascribe many therapeutic properties such as anti-inflammatory, antimicrobial and antioxidant actions to Sida cordifolia Linn. which contains many polyphenols in its roots and seeds.  Evaluation of in vivo antioxidant action in rats of the aqueous extract of Sida cordifolia Linn. roots has been done by introducing potassium dichromate for creating acute and chronic oxidative stress. The Folin- Ciocalteu reagent was utilized for assessing the phenol content which was determined as 27.375 mg Gallic acid/gm. During the acute toxicity tests using mice, no significant adverse signs or symptoms of toxicity were noticed up to the dose of 1500 mg/kg and no mortality was reported until this dosage. The Phosphomolybdate Assay using 3, 6 and 9 mg/ml of research drug concentrations resulted in determination of the Total Antioxidant Capacity that was found to be 15.40 ±9.13, 19.00 ±7.56 and 21.02 ±8.42 gm equivalents of Ascorbic acid. The results of the d-ROMs test which assessed the hydroperoxide levels clearly suggested that introduction of research drug could significantly counter the negative effects of both acute and chronic oxidative stress. Broadly similar findings were obtained by using ELISA kit for assessing ROS values where concurrent administration of the research drug resulted in very significant amelioration in respect of oxidative stress conditions.

Author(s) Details

Dr. Mradu Gupta
Institute of Post Graduate Ayurvedic Education and Research, 294/3/1, APC Road, Kolkata, India.

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In vitro Impact Assessment of Aqueous Extract of Sida cordifolia Linn. Upon Rat Spermatozoa Parameters | Chapter 07 | Emerging Research in Medical Sciences Vol. 1

Sida cordifolia Linn. finds frequent mention in Ayurvedic System of Medicine for many therapeutic properties including beneficial effect upon male reproductive processes. Aim of present in vitro study was to evaluate impact of aqueous extract of Sida cordifolia roots on rat spermatozoa in terms of count, motility & morphology and through HOS study following standard assessment methods to assess any likely reproductive toxicity, spermicidal action, lethality or abnormalities in animal experimentation. Sexually mature (18-22 weeks old) male Wister rats weighing 180 to 260 gm were used. Sperms were collected from cauda epididymis of rat testes and sperm suspension was prepared by mixing them with 2 ml of 5% Sucrose at 37°C, dividing into 4 samples – control (A) and 3 drug treated samples (B, C & D) containing 10, 20 or 30 mg/ml of aqueous extract of Sida cordifolia. The various sperm parameters were observed under Olympus CX41 microscope. Sperm concentration, Progressive and Non-Progressive motility were calculated using the Neubauer Chamber. The viability and morphology of sperm suspensions were assessed using Eosin Y and Nigrosin staining method using 400 X magnification under Phase contrast microscope. Hypo-osmotic swelling (HOS) test was used to evaluate the functional integrity of plasma membrane. Treatment with aqueous extract of Sida cordifolia resulted in no significant adverse impact upon sperm count up to 30 mg/ml dose, suggesting no noticeable spermicidal effect. Progressive motility increased marginally due to intervention of Sida cordifolia in a concentration-dependent manner from 40.00±1.07 in Sample A to 43.50± 2.05 in Sample D. Similarly, percentage of normal sperms and their viability exhibited steady enhancements with increasing concentrations of research drug. The HOS test indicated no adverse structural changes in plasma membrane integrity of sperms up to 30mg/ml level. Thus, in vitro assessment of sperm motility, morphology and viability due to treatment with Sida cordifolia aqueous extract indicated no spermicidal or toxic effect and indicated small but significant enhancement in various parameters which was concentration-dependent.

Author(s) Details

Dr. Mradu Gupta
Institute of Post Graduate Ayurvedic Education and Research, 294/3/1, A. P. C. Road, Kolkata, 700009, India.

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