From Sequence of Tumor Liberated Protein (TLP) to Potential Targets for Diagnosis and Therapy: Advanced Study | Chapter 6 | Innovations in Medicine and Medical Research Vol.2

A preliminary analysis of immunoprecipitation followed by Western Blotting (WB) shows corin and TLP precipitate at the same level (approximately 50 KDa) and are recognized by the same antibodies. In parallel the tests of immunoprecipitation were improved by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 with the aim of obtaining a precipitate containing only the TLP. In fact the partial amino acid sequence of TLP shows a high homology with the sequence of human corin (only one amino acid is different) and is present in lung cancer under different isoforms. It is known that human corin is expressed mostly outside the cells and the protein extract derived from the extracellular medium and from the cells transfected with the plasmid, which over expresses corin, shows several bands analysed on SDS-PAGE that are equivalent to the bands (about 50-100 KDa) observed in the WB analysed by anti-TLP.

Author (s) Details

Giulio Tarro
Beaumont Bonelli Foundation for Cancer Research, Naples, Italy and Committee on Biotechnologies and VirusSphere, World Academy of Biomedical Technologies, UNESCO, Paris, France.

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A Literature Review of Physical Therapy for Pregnant Low Back Pain | Chapter 10 | Emerging Research in Medical Sciences Vol. 3

This study aims to review recently published clinical presentation on the effectiveness and impact of current therapy for low back pain (LBP) during pregnancy. This would help to build an information background and determine the necessity for future research on the development of an effective means for reducing pregnant low back pain.

Author(s) Details

M. Y. Li
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

C. W. Kan
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

A. S. W. Wong
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

Y. L. Kwok
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

J. Yip
Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

S. P. Ng
The Hong Kong Community College, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.

T. H. T. Lao
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

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