TLP Sequenced and Studied for Its Functions with Targets Diagnostic and Therapeutic: Recent Perspective | Chapter 16 | Innovations in Medicine and Medical Research Vol.2

From the first analysis of immuneprecipitation followed by Western Blotting (WB) Corin and TLP seem to precipitate at the same height (approximately 50 KDa) and are recognized by the same antibodies. In parallel the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 are improved with the aim to be able of obtaining a precipitate containing only the TLP. In fact the partial aminoacid sequence of TLP showes a high homology with the sequence of human Corin (only one aminoacid is different) and is present in lung cancer under different isoforms. It is known that human Corin is expressed mostly outside the cells and the protein extract derived from the extracellular medium and from the cells transfected with the plasmid, which overexpresses Corin, showes many more bands analyzed on SDS-PAGE that are equivalent to the bands (about 50-100 KDa) observed in the WB analyzed with anti-TLP. 

Author(s) Details

Giulio Tarro
Beaumont Bonelli Foundation for Cancer Research, Naples, Italy and Committee on Biotechnologies and VirusSphere, World Academy of Biomedical Technologies, UNESCO, Paris, France.

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Development on Tumor Associated Antigen with Specific Target toward Lung Cancer: Recent Developments | Chapter 19 | Innovations in Medicine and Medical Research Vol. 2

Tumor liberated protein (TLP) has been previously described as a TAA (complex) present in the sera from lung cancer patients with early stage disease. Since early detection improves overall survival in lung cancer, identification of screening biomarkers for patients at risk for the development of this disease represents an important target. Starting from the peptide epitope RTNKEASI previously isolated from TLP complexes, we generated a rabbit anti-RTNKEASI serum. This antiserum detected and immunoprecipitated a 55 kDa protein band in the lysate of the lung cancer cell line A549. This protein band was identified as aldehyde dehydrogenase is form 1A1 through mass spectrometry, revealing the molecular nature of at least one component of the previously described TLP complex. 

Author(s) Details

Giulio Tarro
Beaumont Bonelli Foundation for Cancer Research, Naples, Italy and Committee on Biotechnologies and VirusSphere, World Academy of Biomedical Technologies, UNESCO, Paris, France.

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Study on Human Vaccines for Oncogenic Viruses and Perspectives for Tumor Antigens Induced by Virus | Chapter 7 | Innovations in Medicine and Medical Research Vol.2

The Variola major, the virus that causes the smallpox, lethal virus in the 30% of the cases, was eraticated in 1979 in the human species, thanks to a capillary vaccination on global scale. Recently the Word Health Organization (WHO) declared that India and Southeast Asia are polio-free, really a great achivement since the vaccine for polio, an infectious desease that can cause paralysis, was certificated safe and useful only 60 years ago. The vaccine for the virus, responsible for hepatitis B infection HBV, is able to prevent 50% of all liver cancers. Human Papilloma Virus (HPV) have been correlated with the cervical cancer (genotypes 16 and 18 particularly oncogenic in humans): the USA Food and Drug Administration (FDA) in 2006 released the first vaccine against HPV. Finally, the ability of the immune system to recognize a tumor-associated antigen enables the development of a vaccine approach for therapeutic application and represents a main target of this field of research. Long years of research were required for busting new systems to fight cancer. Research is going to obtain the complete sequence by proteomics approaches, in order to achieve adequate antigen preparations that might be used to generate assays for a specific anticancer vaccine. 

Author(s) Details


Giulio Tarro
Beaumont Bonelli Foundation for Cancer Research, Naples, Italy and Committee on Biotechnologies and Virus Sphere, World Academy of Biomedical Technologies, UNESCO, Paris, France.

View Book :- http://bp.bookpi.org/index.php/bpi/catalog/book/161